| 质粒名称: | pC002 - LshC2C2 locus into pACYC184 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 79151 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pACYC184 |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 4724 bp |
| 完整质粒大小: | 10657 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LshC2c2 locus |
| 物种来源: | Leptotrichia shahii |
| 插入大小: | 5933 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pC002 - LshC2C2 locus into pACYC184 was a gift from Feng Zhang (Addgene plasmid # 79151 ; http://n2t.net/addgene:79151 ; RRID:Addgene_79151) |
| 参考文献部分: | C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F. Science. 2016 Jun 2. pii: aaf5573. 10.1126/science.aaf5573 PubMed 27256883 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC002 - LshC2C2 locus into pACYC184 10606 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Endogenous LshC2c2 locus cloned into pACYC184.
ACCESSION .
VERSION .
KEYWORDS pC002 - LshC2C2 locus into pACYC184
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10606)
AUTHORS Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox
DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K,
Regev A, Lander ES, Koonin EV, Zhang F
TITLE C2c2 is a single-component programmable RNA-guided RNA-targeting
CRISPR effector.
JOURNAL Science. 2016 Jun 2. pii: aaf5573.
PUBMED 27256883
REFERENCE 2 (bases 1 to 10606)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10606
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(1475..2665)
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
primer_bind 2294..2313
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS 3062..7231
/codon_start=1
/product="type VI CRISPR effector from Leptotrichia shahii
with RNA-guided RNAse activity (Abudayyeh et al., 2016)"
/label=LshC2c2
/translation="MGNLFGHKRWYEVRDKKDFKIKRKVKVKRNYDGNKYILNINENNN
KEKIDNNKFIRKYINYKKNDNILKEFTRKFHAGNILFKLKGKEGIIRIENNDDFLETEE
VVLYIEAYGKSEKLKALGITKKKIIDEAIRQGITKDDKKIEIKRQENEEEIEIDIRDEY
TNKTLNDCSIILRIIENDELETKKSIYEIFKNINMSLYKIIEKIIENETEKVFENRYYE
EHLREKLLKDDKIDVILTNFMEIREKIKSNLEILGFVKFYLNVGGDKKKSKNKKMLVEK
ILNINVDLTVEDIADFVIKELEFWNITKRIEKVKKVNNEFLEKRRNRTYIKSYVLLDKH
EKFKIERENKKDKIVKFFVENIKNNSIKEKIEKILAEFKIDELIKKLEKELKKGNCDTE
IFGIFKKHYKVNFDSKKFSKKSDEEKELYKIIYRYLKGRIEKILVNEQKVRLKKMEKIE
IEKILNESILSEKILKRVKQYTLEHIMYLGKLRHNDIDMTTVNTDDFSRLHAKEELDLE
LITFFASTNMELNKIFSRENINNDENIDFFGGDREKNYVLDKKILNSKIKIIRDLDFID
NKNNITNNFIRKFTKIGTNERNRILHAISKERDLQGTQDDYNKVINIIQNLKISDEEVS
KALNLDVVFKDKKNIITKINDIKISEENNNDIKYLPSFSKVLPEILNLYRNNPKNEPFD
TIETEKIVLNALIYVNKELYKKLILEDDLEENESKNIFLQELKKTLGNIDEIDENIIEN
YYKNAQISASKGNNKAIKKYQKKVIECYIGYLRKNYEELFDFSDFKMNIQEIKKQIKDI
NDNKTYERITVKTSDKTIVINDDFEYIISIFALLNSNAVINKIRNRFFATSVWLNTSEY
QNIIDILDEIMQLNTLRNECITENWNLNLEEFIQKMKEIEKDFDDFKIQTKKEIFNNYY
EDIKNNILTEFKDDINGCDVLEKKLEKIVIFDDETKFEIDKKSNILQDEQRKLSNINKK
DLKKKVDQYIKDKDQEIKSKILCRIIFNSDFLKKYKKEIDNLIEDMESENENKFQEIYY
PKERKNELYIYKKNLFLNIGNPNFDKIYGLISNDIKMADAKFLFNIDGKNIRKNKISEI
DAILKNLNDKLNGYSKEYKEKYIKKLKENDDFFAKNIQNKNYKSFEKDYNRVSEYKKIR
DLVEFNYLNKIESYLIDINWKLAIQMARFERDMHYIVNGLRELGIIKLSGYNTGISRAY
PKRNGSDGFYTTTAYYKFFDEESYKKFEKICYGFGIDLSENSEINKPENESIRNYISHF
YIVRNPFADYSIAEQIDRVSNLLSYSTRYNNSTYASVFEVFKKDVNLDYDELKKKFKLI
GNNDILERLMKPKKVSVLELESYNSDYIKNLIIELLTKIENTNDTL"
rep_origin 9214..9759
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 9660..9679
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9876..9893
/label=L4440
/note="L4440 vector, forward primer"
promoter 10285..10387
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 10388..441
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(10454..10473)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
541 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
601 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
661 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
721 gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc
781 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg
841 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg
901 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
961 cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg
1021 ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt
1081 tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg
1141 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
1201 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
1261 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg
1321 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
1381 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
1441 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
1501 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
1561 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga
1621 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
1681 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
1741 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
1801 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
1861 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
1921 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
1981 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
2041 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
2101 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
2161 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
2221 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
2281 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
2341 tgatgccggc cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg gtcgccatga
2401 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc
2461 ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta
2521 gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg
2581 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
2641 cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg
2701 ataaactacc gcattaaagc tcgtttaaca gctgggaaaa tgtggaattt gaaacaaatt
2761 atcaggatga aagatttatt gatttggtaa cagaaggcag aacatttaga acaggttcaa
2821 atgggaaaat taagttggat ctaaaggcga aagaaggatt gattttacga aaagtaagga
2881 tagatgggga ttatgagtaa atagaagtag ggagctatgt ttgacggctc cttatttttt
2941 tgagatttaa tattgatgtg gtatactaaa aatggaaaat tgtatttttg attagaaaga
3001 tgtaaaattg atttaattta aaaatatttt attagattaa agtagaaacg aggagattgg
3061 aatgggaaat ttatttggac ataagagatg gtatgaagtt cgtgataaaa aagattttaa
3121 aataaaacgg aaagtaaaag ttaaaaggaa ctatgatgga aacaaataca ttttaaatat
3181 taatgaaaat aataataaag aaaaaattga taataataaa tttattagaa aatatattaa
3241 ttacaaaaaa aatgataata ttcttaaaga atttacaaga aaatttcatg caggaaatat
3301 tctatttaag ttgaaaggta aagaaggaat cataagaata gaaaataatg atgatttttt
3361 ggaaacagaa gaagttgtat tatatataga agcatatgga aaatctgaga aattgaaagc
3421 attggggatt acaaaaaaga aaataataga tgaagcaatt aggcaaggaa taactaaaga
3481 tgataaaaaa atagaaataa agagacaaga gaatgaagaa gaaatagaga tagatatcag
3541 agatgagtat actaataaaa cactaaatga ttgttcaata atattaagaa taatagaaaa
3601 tgatgaatta gaaacaaaaa aatcaattta cgaaattttt aaaaatatta acatgagttt
3661 atataagatt atagaaaaaa taattgaaaa tgaaacagaa aaagtatttg aaaatcgata
3721 ttatgaagaa catttaagag aaaaattatt gaaagatgat aaaatagatg ttattttgac
3781 taattttatg gaaataagag aaaaaataaa aagtaattta gaaattctgg gttttgtaaa
3841 attttatctt aatgttggtg gtgataaaaa gaaatctaaa aataaaaaaa tgcttgttga
3901 aaaaatttta aatataaatg tagatttaac agtagaagat atagctgatt ttgttataaa
3961 agagttagaa ttttggaata ttactaaaag aattgaaaaa gtaaaaaaag ttaataatga
4021 atttcttgaa aagagaagaa atagaacata tataaaatct tatgtactat tagataagca
4081 cgaaaaattt aaaatagaaa gagaaaataa aaaagataaa attgtaaaat tttttgtaga
4141 aaatataaaa aataatagta taaaagaaaa aatagaaaaa attttagcag aatttaaaat
4201 agatgaatta ataaaaaaat tagaaaaaga actaaaaaaa ggaaattgtg atacagaaat
4261 ttttggaata tttaaaaaac attataaagt taattttgat tcgaaaaaat tttcaaagaa
4321 atctgatgaa gaaaaagaac tatataaaat catatatcga tatttgaaag gaagaattga
4381 aaaaatattg gtaaatgaac aaaaagtcag attaaagaaa atggaaaaaa tagaaataga
4441 aaaaatttta aatgaaagta tattatctga aaaaatttta aaaagagtta agcaatatac
4501 gttagagcat attatgtatt tagggaaatt aagacataat gatatagata tgacaacagt
4561 aaatactgat gatttttcta gacttcacgc taaagaggaa ttggatttag aattgattac
4621 tttttttgct tctaccaata tggaattaaa taagattttt tctagagaaa atataaataa
4681 tgatgaaaat atagatttct ttggaggaga tcgagaaaaa aattacgtac ttgataaaaa
4741 aattttaaat tcaaaaataa aaataataag agacttagat tttatagata ataaaaataa
4801 cattacaaat aattttatta ggaaatttac aaagatagga acaaatgaaa gaaacaggat
4861 attacatgcg attagcaagg aaagagattt acaaggaacg caagatgact ataataaagt
4921 cataaatata attcaaaatt taaaaatatc agatgaagaa gtgtcaaaag cattaaattt
4981 agatgtagta tttaaagata aaaaaaatat aattactaaa ataaatgata ttaaaatttc
5041 agaagaaaat aataatgata ttaaatattt accatccttt tctaaagtac ttcctgaaat
5101 attaaattta tataggaata atcctaagaa tgaaccattt gatactatag aaacagaaaa
5161 aatagtatta aatgctttaa tatatgtgaa taaagaattg tataagaaat taattttaga
5221 agatgattta gaagaaaatg aaagtaagaa tatattttta caagaattaa agaaaacttt
5281 aggaaacatt gatgaaatag atgaaaatat tatagaaaat tattataaaa atgctcagat
5341 ttctgcttca aaaggtaata ataaagctat taaaaaatat cagaaaaaag taatagaatg
5401 ttatattgga tatttgagaa aaaattatga ggaacttttt gatttttcag attttaaaat
5461 gaatattcaa gaaattaaga aacaaataaa agacattaat gataataaaa cttacgaaag
5521 aataactgta aagactagtg ataaaactat agtaataaat gatgattttg aatatataat
5581 ttcaatattt gcactgttaa atagtaatgc ggttataaat aaaattagaa atagattttt
5641 tgcaacatca gtttggttaa atacttcaga atatcaaaat attatagata ttttagatga
5701 aataatgcaa ttaaatactt tgagaaatga atgtataact gaaaattgga atcttaattt
5761 agaagaattt attcaaaaaa tgaaagagat agaaaaagac tttgatgatt ttaaaataca
5821 aactaaaaaa gaaattttta ataattatta tgaggatata aaaaataata tattaactga
5881 atttaaagat gatattaatg gttgtgatgt tttagagaaa aaattagaaa aaattgtaat
5941 ttttgatgat gaaactaaat ttgagataga taaaaaatcg aatatattac aagatgaaca
6001 aagaaagtta tcaaacataa ataaaaagga tttgaaaaaa aaggttgatc agtatataaa
6061 agataaagat caggaaataa agagtaaaat attgtgtaga ataatattta attctgattt
6121 tttaaaaaaa tacaaaaaag aaatagataa tttaatagaa gatatggaat cagagaatga
6181 aaataaattt caagaaatat attatcctaa agaacgtaag aatgaattat atatttataa
6241 aaagaattta tttttaaata ttggaaatcc aaattttgat aaaatttatg gattaatttc
6301 aaatgatatt aaaatggctg atgcaaaatt tttatttaat attgatggta aaaatattag
6361 aaaaaataaa atatctgaaa ttgatgcaat attgaaaaat ctaaatgata aactaaatgg
6421 atattctaaa gaatacaaag agaagtatat aaaaaaattg aaagaaaatg atgacttttt
6481 tgcaaaaaat atacaaaata aaaactataa atcatttgaa aaagattata atagagtttc
6541 tgaatacaaa aaaattagag atttggtaga atttaattat ttgaataaaa tagaaagtta
6601 tttgatagat ataaattgga aacttgctat tcaaatggct agatttgaaa gagatatgca
6661 ctatattgta aatgggctaa gagaattagg aattattaag ttaagtggct acaatactgg
6721 aataagtaga gcttatccta agcgtaatgg atctgatggc ttttatacaa caacagcata
6781 ctataaattt tttgatgaag aatcttataa aaaatttgag aaaatatgtt atggatttgg
6841 aattgatttg tctgaaaatt ctgaaattaa taagccggaa aatgaaagta ttagaaacta
6901 tatttcacat ttctatattg taagaaatcc gtttgctgat tacagtattg cagaacaaat
6961 tgatagagtt tctaatttat tatcatatag tacacgttat aataattcaa cttatgcaag
7021 tgtatttgaa gtatttaaaa aagatgtgaa tttagattat gatgaattaa agaaaaaatt
7081 taaacttatc ggtaataatg atatacttga aagattaatg aaacctaaaa aagtttctgt
7141 tttagaatta gaaagttata attctgatta tattaaaaac ttgataatag aacttttaac
7201 aaaaatagaa aatacgaatg atacgttata agaatgaaat ttataaggag tatgattaaa
7261 atgcccaata taattcacat aacaagggct gatgatttgt caatatcgaa taatcagctt
7321 ataatggttg atgaagacaa taatgatgag aaaaataaga tttctttgaa tgatatatct
7381 gcgatagtta ttgaaaactg ctattgtaag attagtgcga ttttgcagtt gaggctggtg
7441 gaaaataaca ttccgattat tatttgcaac gagaaacatc agcctgaaat tcattcgctg
7501 ggattattta atcattttca agtgacttta cgaataaatg agcagattga gtgggaaaaa
7561 gagaaaaagg aaaaattgtg gagcaggata gtggagaata agattgaaaa tcaaagagca
7621 ttgttagagt atcttgaaaa aagtgatatt tccattgaaa gactgaaaac atataaggaa
7681 aacttgaaaa aagatgatgc gagtgcagaa catcaggaag caatagcttc aaggatatat
7741 tttcaggaat tgtatagtaa tagttttaaa agatttgatg aagatggagt gaattctgca
7801 cttaattatg gatatatgat tttaagagct ataatatcat caaagattgt agcaaaagga
7861 tttcatccaa gtttaggatt acatcataag tcacagttta atgcctataa tttttctgat
7921 gatataatag aggtttttcg tcctatggtg gattatcttg tttatatgta caaggatatt
7981 ttgaatgaag tgaaattaag taaggaaata aggcaaaaaa tacttcttgt agcacagcag
8041 aaggtattat ttaacaataa gaaatatgat tttttccaag cggtagacta ttatttggat
8101 agtatacgaa attattttgt aaaagatgaa gaagtgataa ttccgacatt gagcgtgatg
8161 gactatgagt attgagtatt ttggtctgct tatgtatgat tttcctatgc agacagatgt
8221 tgagagattt gaatatagga cttttaggaa aaaattgata gaaaagggat attatcagct
8281 tcaaaaatcg gtatatataa tgaggtcgaa aacaaaagaa aaaatcgaac tggcagaaaa
8341 acagttatca atgctcgctc cagaaaattc atcaatccgt tccattatcc tgacagaaga
8401 tcagtttcaa aaaatgaagg tacttgcagg agaagtgaca atgggagaaa aaatttcaca
8461 aaacaaaata ttggaatttt gaaaaatcaa aaaatatggt ataatagtat agggtggttc
8521 tatattatta ataaagagaa aaataggatt gaaagtattg aaaataaaga caaaatagaa
8581 aaattagaaa taaatatctc atcagcaaga aattaggatg ggatatagac caccccaata
8641 tcgaagggga ctaaaactag attgctgttc taccaagtaa tccatattga tatagaccac
8701 cccaatatcg aaggggacta aaactagatt gctgttctac caagtaatcc atattgatat
8761 agaccacccc aatatcaaag gggactaaaa ctgataacta aaggggaaaa ggtaaaaatg
8821 aggatataga ccaccccaat atcgaagggg actaaaacga caaatctatc tgaataaact
8881 cttctttttc gatatagacc accccaatat cgaaggggac taaaactcta aagaattatc
8941 tattctgtct tttaaattga tatagaccac cccaatatcg aaaagtgata tttaataaaa
9001 ataacttctg agtgagaata aaatttcaat tcttgctcat tttttattgt tttttgaata
9061 tggttgcttg gttgttctga aacgaaaatt tttggagatg ttttaaattt ttaggttgaa
9121 aaaaaataag aattatacta taataaataa ttatgcgatt aggctaacta gagcctaatt
9181 taaatatttt atctgattaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat
9241 ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc
9301 taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct
9361 ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag
9421 tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac
9481 cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
9541 gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc
9601 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg
9661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag
9721 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc
9781 tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag
9841 ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
9901 aatatatcct gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac
9961 atgaagcact tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc
10021 tagcgctgat gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag
10081 gagggacagc tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta
10141 aatcagtaag ttggcagcat cacccgacgc actttgcgcc gaataaatac ctgtgacgga
10201 agatcacttc gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc
10261 caacttttgg cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa
10321 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
10381 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
10441 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
10501 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
10561 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattc
//
